Hamilton PRP-X200 cation exchange HPLC columns are designed for rapid, high resolution separation of alkali and alkaline earth metals. The alkali metals and ammonium are completely resolved in less than five minutes, and the alkaline earth cations separate in under four minutes. Since the mobile phase conditions are different and unique for each of the groups of cations, interferences between these groups are eliminated. The resolution between any two ions in the alkali metal series can be increased or decreased by changing the concentration of methanol in the mobile phase. This unique feature of the PRP-X200 mobile phase/stationary phase interaction allows the chromatographer to focus on the particular ion of interest and reduce possible interference from other ions in the sample.
The stationary phase is a sulfonated poly (styrene-divinylbenzene), so it is stable to all concentrations of organic modifiers as well as strong acids and bases. The rigid spherical polymer phase allows operation up to 5,000 psi. The PRP-X200 columns are designed for use in all of today’s ion chromatographic equipment. Since exchange capacity is low, background signal is low. This allows low detection limits at the highest conductivity detector settings.
Pack Your Own Columns – Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.
Protect Your Investment – Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.
| HPLC Columns | Thomas No. 0470U49 | Thomas No. 0470U50 | Thomas No. 1207L00 |
| Stationary Phase | PRP-X200 | PRP-X200 | PRP-X200 |
| Mode | Cation Exchange | Cation Exchange | Cation Exchange |
| USP Classification | USP L22 | USP L22 | USP L22 |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 10 µm | 10 µm | 10 µm |
| Pore Size | 100 Å | 100 Å | 100 Å |
| Format | Column | Column | Column |
| Hardware Inner Diameter | 4.1 mm | 4.1 mm | 4.6 mm |
| Hardware Length | 150 mm | 250 mm | 250 mm |
| Hardware Material | Stainless Steel | Stainless Steel | PEEK |
| Max. Pressure | 5000 | 5000 | 5000 |
| Exchange Capacity | 35 µeq/gm | 35 µeq/gm | 35 µeq/gm |
| Mobile Phase Limits | pH 1-13. 0-100% aqueous, organic modifier | pH 1-13. 0-100% aqueous, organic modifier | pH 1-13. 0-100% aqueous, organic modifier |
| Buffer Strength | 0.0-0.5N | 0.0-0.5N | 0.0-0.5N |
| Temp Limits | 5-60°C | 5-60°C | 5-60°C |
| Restoration | Inject several times with 100 µL of 1 N nitric acid | Inject several times with 100 µL of 1 N nitric acid | Inject several times with 100 µL of 1 N nitric acid |
| Compound Classification | Organic/Inorganic Cations | Organic/Inorganic Cations | Organic/Inorganic Cations |
| UOM | 1 Each | 1 Each | 1 Each |
| Exchange Capacity/Column | 0.028 meq/column | 0.056 meq/column | 0.042 meq/column |
| Bulk Resin | Thomas No. 1177G10 | Thomas No. 1177G11 |
| Stationary Phase | PRP-X200 | PRP-X200 |
| Mode | Cation Exchange | Cation Exchange |
| Particle Size | 10 µm | 12-20 µm |
| Format | Bulk Resin | Bulk Resin |
| Max. Pressure | 5,000 psi | – |
| Exchange Capacity | 35 µeq/gm | 35 µeq/gm |
| Restoration | Inject several times with 100 µL of 1 N nitric acid | Inject several times with 100 µL of 1 N nitric acid |
| UOM | per gram | per gram Steel |
| Guard Columns | Thomas No. 1177F79 Analytical Guard Column |
Thomas No. 1177G12 Semiprep/Preparative Guard Column |
| Stationary Phase | PRP-X200 | PRP-X200 |
| Mode | Cation Exchange | Cation Exchange |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 10 µm | 12-20 µm |
| Format | Guard Column | Guard Column |
| Hardware Inner Diameter | 2.1 mm | 4.6 mm |
| Hardware Length | 33 mm | 33 mm |
| Guard Column Size | Analytical | Semiprep/Preparative |
| Hardware Material | Stainless Steel | Stainless Steel |
| Max. Pressure | 5,000 psi | 5,000 psi |
| Compound Classification | Organic/Inorganic Cations | Organic/Inorganic Cations |
| UOM | 1 Each | 1 Each |
| Thomas No | Mfr No | Description |
|---|---|---|
| 0470U49 | 79441 | Column PRP-X200 10um |
| 0470U50 | 79442 | Column PRP-X200 10um |
| 1207L00 | 79357 | PRP-X200 10 µm 4.6 x 250 mm PEEK |
| 1177G10 | 79587 | PRP-X200, 10 µm Bulk Resin |
| 1177G11 | 79588 | PRP-X200, 12-20 µm Bulk Resin |
| 1177F79 | 79288 | Analytical Guard Column, PRP-X200 |
| 1177G12 | 79914 | Semiprep/Preparative Guard Column, PRP-X200 |
