Polymeric cation exchange packing for separation of glyphosate and its metabolite in drinking water.
- Faster analysis when compared to competitor’s columns
- Detection levels of less than 10 ppb when using the post column, OPA derivatization technique
- Applicable to other separations, such as inositol and sugar alcohols
- pH stable from 1 to 13
- Two particle sizes: 7 and 12-20 μm
- Five column internal diameters: 2.1 to 21.5 mm
- Two column materials: 316 stainless steel and PEEK
- Analytical and semiprep/preparative guard columns
The PRP-X400 column provides a fast separation for glyphosate and its metabolites. The exchange capacity of the PRP-X400 is greater than that of the PRP-X200, leading to characteristics better suited for the separation of glyphosate. It also performs well in other separations, such as inositol and sugar alcohols. The column does not have to be heated to 65°C and operates well at room temperature, so a column heater is not necessary for this method. PRP-X400 columns do not require the use of methanol in the mobile phase, and they cost much less than other glyphosate columns.
Pack Your Own Columns – Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.
Protect Your Investment – Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.
| PRP-X400 Cation Exchange HPLC Columns, PRP-X400 |
Thomas No. 1216M16 Column, 7µm 250 x 4.1mm |
| Stationary Phase | PRP-X400 |
| Mode | Cation Exchange |
| Packing Material Type | PSDVB/Sulfonic Acid |
| Particle Size | 7 µm |
| Pore Size | N/A |
| Format | Column |
| Hardware Inner Diameter | 4.1 mm |
| Hardware Length | 250 mm |
| Hardware Material | Stainless Steel |
| Max. Pressure | 5000 |
| Exchange Capacity | 2.5 meq/gm |
| Mobile Phase Limits | pH 1-13. 0-100% aqueous, organic modifier |
| Buffer Strength | 0.0-0.5N |
| Temp Limits | 5-60°C |
| Restoration | Inject several times with 100 µL of 0.1 M potassium EDTA |
| Compound Classification | Glyphosate |
| UOM | 1 Each |
| Exchange Capacity/Column | 4.0 meq/column |
| Analytical Guard Column Starter Kits and Cartridge Replacements, PRP-X400 |
Thomas No. 0470U55 Column Starter Kit C-18 |
Thomas No. 0470U59 Cartridge Replacement C-18 Pk5 |
| Stationary Phase | HxSil C18 | HxSil C18 |
| Mode | Reversed-Phase | Reversed-Phase |
| Packing Material Type | Silica | Silica |
| Particle Size | 10 µm | 10 µm |
| Format | Guard Cartridge Kit | Replacement Guard Cartridge |
| Hardware Inner Diameter | 2.1 mm | 2.1 mm |
| Hardware Length | 20 mm | 20 mm |
| Guard Column Size | – | Analytical |
| Hardware Material | Stainless Steel | Stainless Steel |
| Max. Pressure | 5,000 psi | 5,000 psi |
| Mobile Phase Limits | pH 2.0 – 7.5 | pH 2.0 – 7.5 |
| Compound Classification | General Purpose | General Purpose |
| UOM | 1 holder, 2 cartridges | 5/p |
Starter Kits and Cartridge Replacements, PRP-X400 |
Thomas No. 1177F72 Analytical Guard Column Starter Kit |
Thomas No. 1177FF83 Analytical Guard Column |
| Stationary Phase | PRP-X400 | PRP-X400 |
| Mode | Cation Exchange | Cation Exchange |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 7 µm | 7 µm |
| Format | Guard Cartridge Kit | Guard Column |
| Hardware Inner Diameter | 2.1 mm | 2.1 mm |
| Hardware Length | 20 mm | 33 mm |
| Guard Column Size | Semiprep/Preparative | Analytical |
| Hardware Material | Stainless Steel | Stainless Steel |
| Max. Pressure | 5,000 psi | 5,000 psi |
| Mobile Phase Limits | pH 1-13. 0-100% aqueous, organic modifier | _ |
| Compound Classification | Organic/Inorganic Cations | Glyphosate |
| UOM | 1 holder, 2 cartridges | 1 Each |
| Thomas No. 1177F84 7 µm Bulk Resin |
Thomas No. 1177F845 12-20 µm Bulk Resin |
Thomas No. 1177F86 30-50 µm Bulk Resin |
|
| Stationary Phase | PRP-X400 | PRP-X400 | PRP-X400 |
| Mode | Cation Exchange | Cation Exchange | Cation Exchange |
| Particle Size | 7 µm | 12-20 µm | 12-20 µm |
| Pore Size | N/A | N/A | N/A |
| Format | Bulk Resin | Bulk Resin | Bulk Resin |
| Max. Pressure | 5,000 psi | 5,000 psi | 5,000 psi |
| Exchange Capacity | 2.5 meq/gm | 2.5 meq/gm | 2.5 meq/gm |
| Restoration | Inject several times with 100 µL of 0.1 M potassium EDTA | Inject several times with 100 µL of 0.1 M potassium EDTA | Inject several times with 100 µL of 0.1 M potassium EDTA |
| UOM | per gram | per gram | per gram |
| Thomas No | Mfr No | Description |
|---|---|---|
| 0470U55 | 79459 | Column Starter Kit C-18 |
| 0470U59 | 79452 | Cartridge Replacement C-18 Pk5 |
| 1216M16 | 79473 | Column, PRP-X400 7µm 250 x 4.1mm |
| 1177F84 | 79591 | PRP-X400, 7 µm Bulk Resin |
| 1177F85 | 79592 | PRP-X400, 12-20 µm Bulk Resin |
| 1177F86 | 79593 | PRP-X400, 30-50 µm Bulk Resin |
| 1177F72 | 79224 | PRP-X400 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
| 1177F83 | 79290 | Analytical Guard Column, PRP-X400 |
