Analysis of sulfur dioxide in wine, as performed on the HI84100 mini titrator, utilizes a simple sample preparation, a high quality titrant dosing pump, potentiometric endpoint detector, and automatic computations. To maintain the high precision of the titrator, a simple pump calibration procedure is performed with HI84100-55 pump calibration solution. The pump calibration procedure involves the analysis of a known volume of a known solution. The pump calibration procedure should be performed anytime the titrant, tubing or ORP electrode is replaced. Periodic calibrations should be performed in order to accurately determine the free and total sulfur dioxide levels in wine.
The HI84100 determines the free and total sulfur dioxide concentration in wine using the Ripper method. The HI84100 can be used in all wines, including red, which are difficult to test using traditional methods associated with a distinctive color change to determine the endpoint. Excess iodide added to the wine sample reacts with iodate introduced by the titrant to produce iodine:
IO3– + 5I– + 6H+ ? 3I2 + 3H2O
The iodine produced in the sample then reacts with sulfur dioxide in the wine according to the redox reaction below:
H2SO3 + I2 ? H2SO4 + 2HI
Replacement reagents required to operate the HI84100 include:
| Titrant solution | HI84100-50 |
| Alkaline reagent for total SO2 | HI84100-51 |
| Acid reagent for total SO2 | HI84100-52 |
| Acid reagent for free SO2 | HI84100-53 |
| Stabilizer powder packets | HI84100-54 |
| Pump calibration standard | HI84100-55 |
