Viva PFP Propyl Columns (USP L43)

Viva HPLC Columns: 3 or 5 µm particles; 300 Å pore size

• Excellent for separating peptides or proteins
• Rugged, spherical particles with 300 Å pore size
• High proportion of pore/surface area available to large molecules

Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.

The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations.

Particle Size: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 5%
End-Cap: yes
pH Range: 2.5 to 8
Temperature Limit: 80°C
USP Phase Code: L43
Phase Category: fluorophenyl propyl
Ligand Type: pentafluorophenyl propyl