Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The p electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
Application: For Aspartame in a Soft Drink
- Reversed-phase column with p electrons
- Monomeric phenyl, fully endcapped
- Preferential retention of aromatic compounds
- Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules
| Thomas No | Mfr No | Description |
|---|---|---|
| 1205C96 | PH12S03-0502WT | Phenyl, 120A, 3um, 50x2mm Column |
| 1205C97 | PH12S03-0503WT | Phenyl, 120A, 3um, 50x3mm Column |
| 1205C98 | PH12S03-0504WT | Phenyl, 120A, 3um, 50x4mm Column |
| 1205C99 | PH12S03-05Q1WT | Phenyl, 120A, 3um, 50×2.1mm Column |
| 1205D00 | PH12S03-1002WT | Phenyl, 120A, 3um, 100x2mm Column |
| 1205D01 | PH12S03-1003WT | Phenyl, 120A, 3um, 100x3mm Column |
| 1205D02 | PH12S03-1046WT | Phenyl, 120A, 3um, 100×4.6mm Column |
| 1205D03 | PH12S03-10Q1WT | Phenyl, 120A, 3um, 100×2.1mm Column |
| 1205D04 | PH12S03-1502WT | Phenyl, 120A, 3um, 150x2mm Column |
| 1205D05 | PH12S03-1503WT | Phenyl, 120A, 3um, 150x3mm Column |
| 1205D06 | PH12S03-1546WT | Phenyl, 120A, 3um, 150×4.6mm Column |
| 1205D08 | PH12S03-H502WT | Phenyl, 120A, 3um, 35x2mm Column |
| 1205D09 | PH12S03-L502WT | Phenyl, 120A, 3um, 75x2mm Column |
| 1181Z90 | PH12S03-01Q1GC | Phenyl, 3um, 120A, 10×2.1mm Guard Cartridges (requires holder 1205L04) |
| 1181Z91 | PH12S03-0204GC | Phenyl, 3um, 120A, 20x4mm Guard Cartridges (requires holder 1205L05) |
