Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The p electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
Application: For Aspartame in a Soft Drink
- Reversed-phase column with p electrons
- Monomeric phenyl, fully endcapped
- Preferential retention of aromatic compounds
- Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules
| Thomas No | Mfr No | Description |
|---|---|---|
| 1205D83 | PH12S21-0110CC | Phenyl, 120A, 20um, 10x10mm Guard Cartridge Column, Pk/2 |
| 1205D84 | PH12S21-0520WTG | Phenyl, 120A, 20um, 50x20mm Guard Column |
| 1205D85 | PH12S21-0530WTG | Phenyl, 120A, 20um, 50x30mm Guard Column |
| 1205D86 | PH12S21-0551DRG | Phenyl, 120A, 20um, 50x50mm Flanged Guard Column |
| 1205D87 | PH12S21-2520WT | Phenyl, 120A, 20um, 250x20mm Column |
| 1205D88 | PH12S21-2530WT | Phenyl, 120A, 20um, 250x30mm Column |
| 1205D89 | PH12S21-2551DR | Phenyl, 120A, 20um, 250x50mm Flanged Column |
| 1205D90 | PH12S21-5020WT | Phenyl, 120A, 20um, 500x20mm Column |
| 1205D91 | PH12S21-5030WT | Phenyl, 120A, 20um, 500x30mm Column |
| 1205D92 | PH12S21-5051DR | Phenyl, 120A, 20um, 500x50mm Flanged Column |
| 1205D93 | PH30S05-1502WT | Phenyl, 300A, 5um, 150x2mm Column |
