Polymeric 7.5% cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates.
- Compatible with UV and RI detectors
- Hydrogen form columns for USP L17 applications
- Calcium form columns for USP L19 applications
- Lead form columns for USP L34 applications
- One particle size: 9 μm
- Three column internal diameters: 4.1, 7.8 and 10.0 mm
- One column material: 316 stainless steel
- Three forms: calcium, lead and hydrogen
Separate compounds through ligand exchange. HC-75 columns separate compounds through ligand exchange. The 8% cross-linked HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the HC-75 (Hydrogen, Calcium and Lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.
Pack Your Own Columns – Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.
Protect Your Investment – Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.
| Cation Exchange Columns | Thomas No. 0470U71 HC-75 Calcium 305 X 7.8mm | Thomas No. 1216M11 HC-75 9µm CAL 250 x 4.1mm | Thomas No. 1216M12 HC-75 H+F 9µm 305 x 7.8mm |
| Stationary Phase | HC-75 (Ca2+) | HC-75 (Ca2+) | HC-75 (H+) |
| Mode | Cation Exchange | Cation Exchange | Cation Exchange |
| USP Classification | USP L19 | USP L19 | USP L17 |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 9 µm | 9 µm | 9 µm |
| Pore Size | Gel-Type | Gel-Type | Gel-Type |
| Format | Column | Column | Column |
| Hardware Inner Diameter | 7.8 mm | 4.1 mm | 7.8 mm |
| Hardware Length | 305 mm | 250 mm | 305 mm |
| Hardware Material | Stainless Steel | Stainless Steel | Stainless Steel |
| Max. Pressure | 400 psi | 400 psi | 400 psi |
| Exchange Capacity | 5 meq/gm | 5 meq/gm | 5 meq/gm |
| Mobile Phase Limits | 100% Water 0-40% Acetonitrile | 100% Water 0-40% Acetonitrile | 100% Water 0-40% Acetonitrile |
| Buffer Strength | Water | Water | 0.0-0.5N |
| Temp Limits | 25-90°C | 25-90°C | 25-90°C |
| Restoration | 25-90°C | Flush with 1% calcium chloride at 0.1 mL/min overnight. | Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. |
| Compound Classification | Carbohydrates | Carbohydrates | Carbohydrates |
| UOM | 1 Each | 1 Each | 1 Each |
| Exchange Capacity/Column | 32 meq/column | 12 meq/column | 32 meq/column |
| Cation Exchange Columns | Thomas No. 1207L06 HC-75 (Pb2+) 9 µm 7.8 x 100 mm | Thomas No. 1207L07 HC-75 (Pb2+) 9 µm 7.8 x 305 mm | Thomas No. 1207L08 HC-75 (H+) 9 µm 4.1 x 250 mm |
| Stationary Phase | HC-75 (Pb2+) | HC-75 (Pb2+) | HC-75 (H+) |
| Mode | Cation Exchange | Cation Exchange | Cation Exchange |
| USP Classification | USP L34 | USP L17 | USP L17 |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 9 µm | 9 µm | 9 µm |
| Pore Size | Gel-Type | Gel-Type | Gel-Type |
| Format | Column | Column | Column |
| Hardware Inner Diameter | 7.8 mm | 7.8 mm | 7.8 mm |
| Hardware Length | 100 mm | 305 mm | 250 mm |
| Hardware Material | Stainless Steel | Stainless Steel | Stainless Steel |
| Max. Pressure | 400 psi | 400 psi | 400 psi |
| Exchange Capacity | 5 meq/gm | 5 meq/gm | 5 meq/gm |
| Mobile Phase Limits | 100% Water 0-40% Acetonitrile | 100% Water 0-40% Acetonitrile | 100% Water 0-40% Acetonitrile |
| Buffer Strength | Water | Water | 0.0-0.5N |
| Temp Limits | 25-90°C | 25-90°C | 25-90°C |
| Restoration | Flush with 1% lead nitrate at 0.1 mL/min overnight. | Flush with 1% calcium chloride at 0.1 mL/min overnight. | Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. |
| Compound Classification | Carbohydrates | Carbohydrates | Carbohydrates |
| UOM | 1 Each | 1 Each | 1 Each |
| Exchange Capacity/Column | – | – | – |
| Thomas No. 1177G24 | Thomas No. 1177G25 | Thomas No. 1177G26 | |
| Stationary Phase | HC-75 (Ca2+) | HC-75 (H+) | HC-75 (Pb2+) |
| Mode | Cation Exchange | Cation Exchange | Cation Exchange |
| USP Classification | USP L19 | USP L17 | USP L34 |
| Particle Size | 9 µm | 9 µm | 9 µm |
| Pore Size | Gel-Type | Gel-Type | Gel-Type |
| Format | Bulk Resin | Bulk Resin | Bulk Resin |
| Exchange Capacity | 5 meq/gm | 5 meq/gm | 5 meq/gm |
| Restoration | Flush with 1% calcium chloride at 0.1 mL/min overnight. | Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. | Flush with 1% lead nitrate at 0.1 mL/min overnight. |
| UOM | per gram | per gram | per gram |
| Semiprep/Preparative Guard Column | Thomas No. 1177G27 (Semiprep/Preparative Guard Column, Hydrogen Form) | Thomas No. 1177G28 (Semiprep/Preparative Guard Column, Carbonate Form) |
| Stationary Phase | PRP-X400 | PRP-X500 |
| Mode | Cation Exchange | Cation Exchange |
| Packing Material Type | PSDVB/Sulfonic Acid | PSDVB/Sulfonic Acid |
| Particle Size | 12-20 µm | 12-20 µm |
| Format | Guard Column | Guard Column |
| Hardware Inner Diameter | 4.6 mm | 4.6 mm |
| Hardware Length | 33 mm | 33 mm |
| Guard Column Size | Semiprep/Preparative | Semiprep/Preparative |
| Hardware Material | Stainless Steel | Stainless Steel |
| Max. Pressure | 400 psi | 400 psi |
| Compound Classification | Carbohydrates | Carbohydrates |
| UOM | 1 Each | 1 Each |
| Thomas No | Mfr No | Description |
|---|---|---|
| 0470U71 | 79436 | Column HC-75 Calcium 305 X 7.8mm |
| 1216M11 | 79431 | HC-75 9µm CAL 250 x 4.1mm |
| 1216M12 | 79544 | Column HC-75 H+F 9µm 305 x 7.8mm |
| 1207L06 | 79240 | HC-75 (Pb2+) 9 µm 7.8 x 100 mm |
| 1207L07 | 79438 | HC-75 (Pb2+) 9 µm 7.8 x 305 mm |
| 1207L08 | 79476 | HC-75 (H+) 9 µm 4.1 x 250 mm |
| 1177G24 | 79709 | HC-75 (Ca 2+), 9 µm Bulk Resin |
| 1177G25 | 79711 | HC-75 (H+), 9 µm Bulk Resin |
| 1177G26 | 79712 | HC-75 (Pb 2+), 9 µm Bulk Resin |
| 1177G27 | 79917 | Semiprep/Preparative Guard Column, Hydrogen Form |
| 1177G28 | 79919 | Semiprep/Preparative Guard Column, Carbonate Form |
